A Disseminated Intravascular Coagulation Animal Model: How Compound Danshen Dripping Pills Work and What They Do

Abstract

Purpose: Using transcriptome analysis, we want to learn how compound Danshen dripping pills (CDDP) affect diffuse intravascular coagulation (DIC) and what mechanisms CDDP may work by. Research Tools and Procedures: We carried out DIC animal models with various inducers and studied the impact of CDDP on many parameters, including survival rate, blood flow to the hepatic and gastric fundi, aberrant prothrombin time (PT), fibrinogen (FIB) concentration, inflammatory factors, and the damage index for the liver and kidneys. After that, confirmation of the primary target genes was done using whole-blood transcriptome sequencing. The results showed that CDDP increased the efficiency of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase, improved blood flow in the stomach and liver, increased PT and FIB values, decreased the elevated serum levels of interleukin-10 and tumor necrosis factor-alpha, and increased blood urea nitrogen and creatinine. Additionally, it improved the survival rate of the animals used in the study. According to research using whole-blood transcriptome sequencing, CDDP may control inflammatory response in the immune system, oxidative stress, activation and aggregation of platelets, and cell death in DIC. The model animals' aberrant expression of Sqstml, Ctsd, Mylk2, and Nfkbib was further enhanced by CDDP, as demonstrated by quantitative polymerase chain reaction (qPCR). Results: CDDP protects primary organs in DIC models by reducing inflammatory factor expression, increasing organ blood flow, and correcting coagulation anomalies. Not only that, but CDDP enhanced the outcome of DIC animal models by boosting the expression of important genes associated with the illness, namely Sqstml and Nfkbib.