By inhibiting NLRP3 inflammasome via autophagy activation, carnosol improves sevoflurane-induced cognitive impairment in old rats.

Abstract

Specifically, we want to learn how carnosol works and how it affects postoperative cognitive dysfunction
(POCD). Methods: The sevoflurane (SEV) paradigm of cognitive impairment in rats was developed. We
used a dihydroethidium (DHE) test to measure the impact of carnosol on ROS levels in rats that had been
produced by sevoflurane. The impact of carnosol on mitochondrial damage was evaluated using
adenosine triphosphate (ATP) generation and immunoblot tests. Cognitive impairment in the rats was
assessed by a water maze experiment. The immunoblot test was used to evaluate the action mechanism.
The results showed that carnosol inhibited ROS generation and mitochondrial damage caused by
sevoflurane in rats, and it also triggered autophagy. Carnosol also inhibited SEV-induced cognitive
impairment via activating autophagy and SEV-induced NLRP3 inflammasome activation, respectively.
Conclusion: Carnosol, by inhibiting the NLRP3 inflammasome, improves SEV-stimulated cognitive
impairment and may one day be used as a treatment for cognitive impairment. Carnosol, Sevoflurane
(SEV), NLRP3 inflammasome, and autophagy are some of the terms used to describe this phenomenon