Pharmacological Research Antimicrobial Activity of Vitex Negundo Linn. (Nirgundi) Leaves Extract

Abstract

Background: Antibiotic resistance in human pathogens has been more widespread during the last
several years. This necessitates the development of antibacterial medications that are not based on
antibiotics. Aims: in vitro testing of the antibacterial properties on both f and g gramme of Vitex
negundo Linn. (Nirgundi) organisms with a gramme positive and a gramme negative stain
Material and Methods: To test the antimicrobial activity of Vitex negundo Linn. leaf oethanol
extracts against three strains of bacteria obtained from the American Type Culture Collection (ATCC
25922, ATCC 25923, and ATCC 27736), researchers used the well diffusion technique (ATCC). The
concentrations of 20 mg/ml, 40 mg/ml, and 60 mg/ml were used in this investigation. Leaf extract
concentrations of up to 100 mg/ml were utilised.
Results: All of the bacterial pathogens investigated were shown to have antibacterial properties.
Highest ZOI recorded for S. aureus was 15 mm at 80 and 100 mg/ml, respectively, whereas the
maximum ZOI was 12 mm for E. coli and 11 mm for K. pneumoniae at 100 mg/ml for the other two
pathogens.
Conclusion: A significant antibacterial effect was found for S aureus in the Vitex negundo Linn. leaf
extract when compared to all the other microorganisms.